A coronaviruses assembly blocker is meant to target the viral virulence factor

Cells were infected with SARS-COV-2–mNeonGreen virus (At a final MOI of 0.1 TCID50 per cell) and compounds were added 0.5, 0, 2, 4, 6 and 8 h after infection. The samples were prepared in a 384-well plate with 0.5 mg ml1 purified recombinant SARS-CoV-2 M and 100 M of JNJ-9676 in 20 mM HEPES pH 7.5, 150 mM 0.05% GD, 0.0001% CHS, and 1% DMSO.