Blood and brain barrier are affected by Glycocalyx dysregulation
A single embryo was collected at the 8cell and blastocyst stage and a singleembryo was stained toconfirm the KD or marker gene expression. Wild-type ES cells were cultured at 37 C with 5% CO2 and were maintained on 0.1% gelatin-coated plates in high-glucose DMEM culture medium containing 15% fetal bovine serum (invitrogen), 1% penicillin-streptomycin (Hyclone) and 1% MEM nonessential amino acids (Cellgro).
The Trump Administration has a live update regarding the Mineral Deal
US Treasury Secretary Scott Bessent has said he thinks that an American financial interest in the reserves of rare earth minerals is a better security guarantee than a country without one. This comes after President Donald Trump said he won’t guarantee Ukraine’s “safety as part of mineral deal” with the US. Bessent added that he called it an economic security guarantee.
What science photos do that artificial intelligence can’t do?
A photo of Nobel laureate Moungi Bawendi’s nanocrystals in vials, fluorescing at different wavelengths, when excited with UV light has been created using a photo-editing app. The app AlphaFold uses structural models that are turned into images by people and creates the image by using a ” diffuse model” which is a complex computation.
It’s an attack on science everywhere in Trump 2.0
US President Donald Trump signed an executive order to “eliminate all programmes that promote diversity, equity and inclusion”. This comes after he signed two other executive orders, one to scrap federal programmes that promote diversity, equity and inclusion and another to cancel federal funding for international climate change projects. Around one million women around the world have lost access to contraceptive care.
Did the human gene make mice squeak differently?
Scientists have discovered that a human version of the NOVA1 gene is present in nearly every human on Earth. The variant is different from Neanderthals and another extinct human group called Denisovans, as well as other mammals. Researchers used CRISPR gene editing to engineer mice carrying the human version of NOVA1 to study the effects of the change.
Doge can access the data while the judge rules
A US judge declined to block Tesla CEO Elon Musk and his Department of Government Efficiency (DOGE) from accessing or transferring data from seven government agencies. The judge said that DOGE’s actions violate the Privacy Act of 1974. The Privacy Act prohibits an agency from disclosing someone’s records unless that person approves in writing or the agency meets one of the law’s 12 exceptions.
Both the U.S. and Russia discussed an end to the war without Kyiv
Ahead of President Donald Trump’s Ukrainian summit with Russian President Vladimir Putin, a French expert said that European leaders hoped that the US and Europe could work together under the new Trump administration. Elie Tenenbaum, head of the Security Studies Center at the French Institute for International Relations, added that the gulf between the US and Europe on the Ukraine war crystallized for Europeans this past weekend.
There will be protests on Presidents Day
Hundreds of people across the US protested against Tesla CEO Elon Musk on Presidents’ Day by chanting, “Don’t buy swasticars” and “Elon Musk can go to Mars; we don’t need his Nazi cars.” The protests were held in more than half of the states including California, Colorado, Tennessee, Massachusetts, Vermont, New Hampshire, Connecticut and Hawaii.
3 top US prosecutors quit after the NYC Mayor’s corruption case was dropped
Emil Bove, the co-chief of the US Attorney’s Office for the Southern District of New York, helped FBI investigate and arrest the rioters who stormed the US Capitol on January 6 last year. Bove also led efforts by federal prosecutors in Manhattan to help FBI aggressively investigate the rioters from New York region. Bove and Kevin Driscoll worked on the same case.
UM171 glues a CRL3–HDAC1/2 assembly to degrade corepressors
Our study used y1 ion-based technology for the extraction of TMT reporter ion intensities and the generation of mean-centred intensities. We also corrected the raw intensities by the isotopic distribution of each labelling (for example, TMT126 generates 91.8%, 7.9% and 0.3% of 126, 127 and 128 m/z ions, respectively). We also calculated the mean-centred intensities across samples (for example, relative intensities between sample and mean).