Blood and brain barrier are affected by Glycocalyx dysregulation

A single embryo was collected at the 8cell and blastocyst stage and a singleembryo was stained toconfirm the KD or marker gene expression. Wild-type ES cells were cultured at 37 C with 5% CO2 and were maintained on 0.1% gelatin-coated plates in high-glucose DMEM culture medium containing 15% fetal bovine serum (invitrogen), 1% penicillin-streptomycin (Hyclone) and 1% MEM nonessential amino acids (Cellgro).